Calcium ions (Ca²⁺) are essential for numerous cellular processes, including the regulation of mitochondrial function. Mitochondrial calcium (mCa²⁺) enhances mitochondrial metabolism, thereby increasing ATP synthesis. However, excessive mCa²⁺ can lead to ischemic/reperfusion (IR) injury and heart failure (HF), resulting in metabolic disturbances and loss of cardiac myocytes. It is crucial to understand the precise regulation of mCa²⁺ uptake in both physiological and pathological contexts. Our research laboratory, located within the Department of Cell and Molecular Biology at Rowan-Virtua School of Osteopathic Medicine, is dedicated to elucidating the mechanisms governing mCa²⁺ uptake, with the aim of understanding the underlying mechanisms of IR injury and HF. We invite collaborators to join our investigation into the relationship between ionic dysregulation and mitochondrial dysfunction, with the goal of developing effective therapeutic strategies to combat heart failure. With over a decade of experience in manipulating mitochondrial ion homeostasis and signaling pathways, we are committed to contributing to the resolution of mechanistic and translational questions associated with mitochondrial dysfunction in cardiac diseases. Our laboratory at Rowan University School of Osteopathic Medicine has been awarded an R01 grant from the National Heart, Lung, and Blood Institute (NHLBI). Our research endeavors focus on understanding the complexities of these issues and bridging the gap between laboratory findings and clinical applications.