Publications

  • Shcherbik Lab

Publications

Publications

  1. Smethurst DGJ, Shcherbik N. Interchangeable utilization of metals: New perspectives on the impacts of metal ions employed in ancient and extant biomolecules. J. Biol. Chem. 2021. Oct 31: 297(6):101374.
  2. Trainor BM, Komar AA, Pestov DG, Shcherbik N. Cell-free Translation: Preparation and Validation of Translation-competent Extracts from Saccharomyces cerevisiae. BioProtocols. 2021. Sep 20;11(18):e4093.doi: 10.21769/BioProtoc.4093.
  3. Brandon M. Trainor, Dimitri G. Pestov and Natalia Shcherbik. Development, validation, and application of the ribosome separation and reconstitution system for protein translation in vitro. RNA. 2021 Aug 27;rna.078852.121. doi: 10.1261/rna.078852.121
  4. Wang Y, Fang S, Chen G, Ganti R, Chernova TA, Zhou L, Duong D, Kiyokawa H, Li M, Zhao B, Shcherbik N, Chernoff YO, Yin J. Regulation of endocytosis and prion-chaperoning machineries by yeast E3 ubiquitin ligase Rsp5 as revealed by orthogonal ubiquitin transfer. Cell Chem Biol. 2021 Feb 23:S2451-9456(21)00057-X. doi: 10.1016/j.chembiol.2021.02.005. 
  5. Trainor BM, Ghosh A, Pestov DG, Hellen CUT, Shcherbik N. A translation enhancer element from black beetle virus engages yeast eIF4G1 to drive cap-independent translation initiation. Scientific Reports. 2021. Jan 28; 11(1): 2461. doi: 10.1038/s41598-021-82025-6
  6. Daniel G J Smethurst, Nikolay Kovalev, Erica R McKeeenzie, Dimitri G Pestov, Natalia Shcherbik. Iron-mediated degradation of ribosomes under oxidative stress is attenuated by manganese. JBC. 2020, Oct 9, doi: 10.1074/jbc.RA120.015025
  7. Ghosh A,Shcherbik N. Cooperativity between the Ribosome-Associated Chaperone Ssb/RAC and the ubiquitin-ligase Ltn1 in ubiquitination of nascent polypeptides. IJMS. 2020, Sept 17; 21(18): E6815.
  8. Santerre M, Arjona SP, Allen CN,Shcherbik N, Sawaya BE. Why do SARS-CoV-2 NSPs rush to the ER? J Neurology. 2020, Sept 1: 1-10
  9. Chernova TA, Yang Z, Karpova TS, Shanks JR,Shcherbik N, Wilkinson KD, Chernoff YO. Aggregation and prion-induced properties of the G-protein Gamma subunit Ste18 are regulated by membrane association. IJMS. 2020, July 16; 21(14): 5038.
  10. Ghosh A, Williams LD, Pestov DG and Shcherbik N. Proteotoxic stress promotes entrapment of ribosomes and misfolded proteins in a shared cytosolic compartments. Nucleic Acids Res. 2020, Apr 17; 48(7): 3888-3905.
  11. Ghosh A and Shcherbik N. Effects of Oxidative Stress on Protein Translation: Implications for Cardiovascular Diseases. Int J Mol Sci. 202 Apr 11; 21(8) 
  12. Shcherbik N and Pestov DG. The Impact of Oxidative Stress on Ribosome: From Injury to Regulation. Cells. 2019 Nov 2; 8(11). 
  13.  Tatiana A. Chernova, Zhen Yang, Tatiana S. Karpova, John R. Shanks, Natalia Shcherbik, Keith D. Wilkinson, Yury O. Chernoff. Aggregation and Prion-Inducing Properties of the G-Protein Gamma Subunit Ste18 are Regulated by Association with the Plasma Membrane. Int J Mol Sci., accepted on July 9th, 2020.
  14. Zinskie JA, Ghosh A, Trainor BM, Shedlovskiy D, Pestov DG, Shcherbik N. Iron-dependent cleavage of ribosomal RNA during oxidative stress in the yeast Saccharomyces cerevisiae. J Biol Chem, 293(37): 14237-48, September 2018
  15. Shedlovskiy D, Shcherbik N, Pestov DG. One-step hot formamide extraction of RNA from Saccharomyces cerevisiae. RNA Biol, 14(12): 1722-6, December 2017.
  16. Shedlovskiy D, Zinskie JA, Gardner E, Pestov DG, Shcherbik N. Endonucleolytic cleavage in the expansion segment 7 of 25S rRNA is an early marker of low-level oxidative stress in yeast. J Biol Chem, 292(45): 18469-85, November 2017.
  17. Shcherbik N, Chernova TA, Chernoff YO, Pestov DG. Distinct types of translation termination generate substrates for ribosome-associated quality control. Nucleic Acids Res, 44(14): 6840-52, August 2016.
  18. Wang M, Parshin AV, Shcherbik N, Pestov DG. Reduced expression of the mouse ribosomal protein Rpl17 alters the diversity of mature ribosomes by enhancing production of shortened 5.8S rRNA. RNA, 21(7): 1240-8, July 2015.
  19. Shcherbik N. Golgi-mediated glycosylation determines residency of the T2 RNase Rny1p in Saccharomyces cerevisiae. Traffic, 14(12): 1209-27, December 2013.
  20. Pestov DG, Shcherbik N. Rapid cytoplasmic turnover of yeast ribosomes in response to rapamycin inhibition of TOR. Mol Cell Biol, 32(11): 2135-44, Jun 2012.
  21. Shcherbik N, Pestov DG. The ubiquitin ligase Rsp5 is required for ribosome stability in Saccharomyces cerevisiae. RNA, 17(8): 1422-8, Aug 2011.
  22. Shcherbik N, Pestov DG. Ubiquitin and ubiquitin-like proteins in the nucleolus: multitasking tools for a ribosome factory. Genes Cancer, 1(7): 681-9,Jul 2010.
  23. Shcherbik N, Wang M, Lapik YR, Srivastava L, Pestov DG. Polyadenylation and degradation of incomplete RNA polymerase I transcripts in mammalian cells. EMBO Rep, 11(2): 106-11, Feb 2010.
  24. Bhattacharya S, Shcherbik N, Vasilescu J, Smith JC, Figeys D, Haines DS. Identification of lysines within membrane-anchored Mga2p120 that are targets of Rsp5p ubiquitination and mediate mobilization of tethered Mga2p90. J Mol Biol, 385(3): 718-25, Jan 2009.
  25. Shcherbik N, Haines DS.  Cdc48pNpl4p/Ufd1p binds and segregates heterodimeric membrane anchored/tethered complexes via a polyubiquitin signal present on the anchors.  Molecular Cell, 25(3): 385-97, Feb 2007.
  26. Shcherbik N, Kee Y, Lyon N, Huibregtse JM, Haines DS.  A single PXY motif located within the carboxy-terminus of Spt23p and Mga2p mediates a physical and functional interaction with ubiquitin ligase Rsp5p.  J Biol Chem, 279(51): 53892-8, Dec 2004.
  27. Shcherbik N, Haines DS. Ub on the move. J Cell Biochem, 93(1): 11-9, Sep 2004.
  28. Shcherbik N, Zoladek T, Nickels JT, Haines DS.  Rsp5p is required for ER-bound Mga2p120 poly-ubiquitination and release of the processed/tethered transactivator Mga2p90.  Curr Biol, 13(14): 1227-1233, Jul 2003.
  29. Gajewska B, Shcherbik N, Oficjalska D, Haines DS, Zoladek T.  Functional analysis of the human orthologue of the RSP5-encoded ubiquitin protein ligase,  hNEDD4, in yeast.  Curr Genet, 43(1): 1-10, Apr 2003.
  30. Shcherbik N, Kumar S, Haines DS. Substrate proteolysis is inhibited by dominant-negative Nedd4 and Rsp5 mutants harboring alterations in WW domain 1.  J Cell Sci, 115(Pt5): 1041-8, Mar 2002.
  31. Darbinian N, Gallia GL, Kundu M, Shcherbik N, Tretiakova A, Giordano A, Khalili K. Association of Pur alpha and E2F-1 suppresses transcriptional activity of E2F-1. Oncogene, 18(46): 6398-402, Nov 1999.
  32. Tretiakova A, Gallia GL, Shcherbik N, Jameson B, Johnson EM, Amini S, Khalili K. Association of Pur alpha with RNAs homologous to 7 SL determines its binding ability to the myelin basic protein promoter DNA sequence. J Biol Chem, 273(35): 22241-7, Aug 1998.
  33. Safronov IV, Shcherbik NV, Khodyreva SN, Vlasov VA, Dobrikov MI, Shishkin GV, Lavrik OI. New photoreactive N(4)-substituted dCTP analogues: synthesis, photochemical characteristics, and substrate properties in HIV-1 reverse transcriptase catalyzed DNA synthesis. Bioorg Khim, 23(7): 576-85, Jul 1997.
  34. Shcherbik NV, Khodyreva SN, Vlasov VA, Dobrikov MI, Dymshits GM, Lavrik OI. Photoaffinity modification of human immunodeficiency virus reverse transcriptase with an analog of deoxyuridine-5'-triphosphate, containing an arylazide group. Mol Biol (Mosk), 31(2): 344-52, Mar 1997.