Publications
Publications
Publications
Smethurst DGJ, Shcherbik N . Interchangeable utilization of metals: New perspectives on the impacts of metal ions employed in ancient and extant biomolecules . J. Biol. Chem. 2021. Oct 31: 297(6):101374.
Trainor BM , Komar AA, Pestov DG, Shcherbik N . Cell-free Translation: Preparation and Validation of Translation-competent Extracts from Saccharomyces cerevisiae. BioProtocols. 2021. Sep 20;11(18):e4093.doi: 10.21769/BioProtoc.4093.
Brandon M. Trainor, Dimitri G. Pestov and Natalia Shcherbik. Development, validation, and application of the ribosome separation and reconstitution system for protein translation in vitro . RNA. 2021 Aug 27;rna.078852.121. doi: 10.1261/rna.078852.121
Wang Y, Fang S, Chen G, Ganti R, Chernova TA, Zhou L, Duong D, Kiyokawa H, Li M, Zhao B, Shcherbik N , Chernoff YO, Yin J. Regulation of endocytosis and prion-chaperoning machineries by yeast E3 ubiquitin ligase Rsp5 as revealed by orthogonal ubiquitin transfer. Cell Chem Biol. 2021 Feb 23:S2451-9456(21)00057-X. doi: 10.1016/j.chembiol.2021.02.005.
Trainor BM, Ghosh A, Pestov DG , Hellen CUT, Shcherbik N. A translation enhancer element from black beetle virus engages yeast eIF4G1 to drive cap-independent translation initiation. Scientific Reports. 2021. Jan 28; 11(1): 2461. doi: 10.1038/s41598-021-82025-6
Daniel G J Smethurst, Nikolay Kovalev, Erica R McKeeenzie, Dimitri G Pestov, Natalia Shcherbik. Iron-mediated degradation of ribosomes under oxidative stress is attenuated by manganese . JBC. 2020, Oct 9, doi: 10.1074/jbc.RA120.015025
Ghosh A,Shcherbik N. Cooperativity between the Ribosome-Associated Chaperone Ssb/RAC and the ubiquitin-ligase Ltn1 in ubiquitination of nascent polypeptides . IJMS. 2020, Sept 17; 21(18): E6815.
Santerre M, Arjona SP, Allen CN,Shcherbik N , Sawaya BE. Why do SARS-CoV-2 NSPs rush to the ER? J Neurology. 2020, Sept 1: 1-10
Chernova TA, Yang Z, Karpova TS, Shanks JR,Shcherbik N , Wilkinson KD, Chernoff YO. Aggregation and prion-induced properties of the G-protein Gamma subunit Ste18 are regulated by membrane association . IJMS. 2020, July 16; 21(14): 5038.
Ghosh A, Williams LD, Pestov DG and Shcherbik N . Proteotoxic stress promotes entrapment of ribosomes and misfolded proteins in a shared cytosolic compartments . Nucleic Acids Res. 2020, Apr 17; 48(7): 3888-3905.
Ghosh A and Shcherbik N . Effects of Oxidative Stress on Protein Translation: Implications for Cardiovascular Diseases . Int J Mol Sci. 202 Apr 11; 21(8)
Shcherbik N and Pestov DG. The Impact of Oxidative Stress on Ribosome: From Injury to Regulation . Cells. 2019 Nov 2; 8(11).
Tatiana A. Chernova, Zhen Yang, Tatiana S. Karpova, John R. Shanks, Natalia Shcherbik , Keith D. Wilkinson, Yury O. Chernoff. Aggregation and Prion-Inducing Properties of the G-Protein Gamma Subunit Ste18 are Regulated by Association with the Plasma Membrane. Int J Mol Sci., accepted on July 9th, 2020.
Zinskie JA, Ghosh A, Trainor BM, Shedlovskiy D, Pestov DG, Shcherbik N . Iron-dependent cleavage of ribosomal RNA during oxidative stress in the yeast Saccharomyces cerevisiae . J Biol Chem , 293(37): 14237-48, September 2018
Shedlovskiy D, Shcherbik N , Pestov DG. One-step hot formamide extraction of RNA from Saccharomyces cerevisiae. RNA Biol , 14(12): 1722-6, December 2017.
Shedlovskiy D, Zinskie JA, Gardner E, Pestov DG, Shcherbik N . Endonucleolytic cleavage in the expansion segment 7 of 25S rRNA is an early marker of low-level oxidative stress in yeast. J Biol Chem , 292(45): 18469-85, November 2017.
Shcherbik N , Chernova TA, Chernoff YO, Pestov DG. Distinct types of translation termination generate substrates for ribosome-associated quality control. Nucleic Acids Res , 44(14): 6840-52, August 2016.
Wang M, Parshin AV, Shcherbik N , Pestov DG. Reduced expression of the mouse ribosomal protein Rpl17 alters the diversity of mature ribosomes by enhancing production of shortened 5.8S rRNA . RNA , 21(7): 1240-8, July 2015.
Shcherbik N . Golgi-mediated glycosylation determines residency of the T2 RNase Rny1p in Saccharomyces cerevisiae . Traffic , 14(12): 1209-27, December 2013.
Pestov DG, Shcherbik N . Rapid cytoplasmic turnover of yeast ribosomes in response to rapamycin inhibition of TOR. Mol Cell Biol , 32(11): 2135-44, Jun 2012.
Shcherbik N, Pestov DG. The ubiquitin ligase Rsp5 is required for ribosome stability in Saccharomyces cerevisiae . RNA, 17(8): 1422-8, Aug 2011.
Shcherbik N , Pestov DG. Ubiquitin and ubiquitin-like proteins in the nucleolus: multitasking tools for a ribosome factory . Genes Cancer , 1(7): 681-9,Jul 2010.
Shcherbik N, Wang M, Lapik YR, Srivastava L, Pestov DG. Polyadenylation and degradation of incomplete RNA polymerase I transcripts in mammalian cells. EMBO Rep, 11(2): 106-11, Feb 2010.
Bhattacharya S, Shcherbik N , Vasilescu J, Smith JC, Figeys D, Haines DS. Identification of lysines within membrane-anchored Mga2p120 that are targets of Rsp5p ubiquitination and mediate mobilization of tethered Mga2p90 . J Mol Biol , 385(3): 718-25, Jan 2009.
Shcherbik N, Haines DS. Cdc48pNpl4p/Ufd1p binds and segregates heterodimeric membrane anchored/tethered complexes via a polyubiquitin signal present on the anchors . Molecular Cell , 25(3): 385-97, Feb 2007.
Shcherbik N, Kee Y, Lyon N, Huibregtse JM, Haines DS. A single PXY motif located within the carboxy-terminus of Spt23p and Mga2p mediates a physical and functional interaction with ubiquitin ligase Rsp5p . J Biol Chem , 279(51): 53892-8, Dec 2004.
Shcherbik N , Haines DS. Ub on the move . J Cell Biochem , 93(1): 11-9, Sep 2004.
Shcherbik N , Zoladek T, Nickels JT, Haines DS. Rsp5p is required for ER-bound Mga2p120 poly-ubiquitination and release of the processed/tethered transactivator Mga2p90 . Curr Biol , 13(14): 1227-1233, Jul 2003.
Gajewska B, Shcherbik N, Oficjalska D, Haines DS, Zoladek T. Functional analysis of the human orthologue of the RSP5-encoded ubiquitin protein ligase, hNEDD4 , in yeast . Curr Genet , 43(1): 1-10, Apr 2003.
Shcherbik N, Kumar S, Haines DS. Substrate proteolysis is inhibited by dominant-negative Nedd4 and Rsp5 mutants harboring alterations in WW domain 1 . J Cell Sci , 115(Pt5): 1041-8, Mar 2002.
Darbinian N, Gallia GL, Kundu M, Shcherbik N, Tretiakova A, Giordano A, Khalili K. Association of Pur alpha and E2F-1 suppresses transcriptional activity of E2F-1 . Oncogene , 18(46): 6398-402, Nov 1999.
Tretiakova A, Gallia GL, Shcherbik N, Jameson B, Johnson EM, Amini S, Khalili K. Association of Pur alpha with RNAs homologous to 7 SL determines its binding ability to the myelin basic protein promoter DNA sequence . J Biol Chem , 273(35): 22241-7, Aug 1998.
Safronov IV, Shcherbik NV , Khodyreva SN, Vlasov VA, Dobrikov MI, Shishkin GV, Lavrik OI. New photoreactive N(4)-substituted dCTP analogues: synthesis, photochemical characteristics, and substrate properties in HIV-1 reverse transcriptase catalyzed DNA synthesis. Bioorg Khim , 23(7): 576-85, Jul 1997.
Shcherbik NV , Khodyreva SN, Vlasov VA, Dobrikov MI, Dymshits GM, Lavrik OI. Photoaffinity modification of human immunodeficiency virus reverse transcriptase with an analog of deoxyuridine-5'-triphosphate, containing an arylazide group . Mol Biol (Mosk) , 31(2): 344-52, Mar 1997.